Fig. 3.

CD24 knock-down mediates gene regulation and reduces STAT3 protein phosphorylation and activity. a Identification of a CD24-dependent target genes signature (OPG, STC-1, MMP-7, and STAT3) by quantitative RT-PCR analysis of mRNAs isolated after siCD24-mediated knock-down. Note that OPG, STC-1, and MMP-7 genes are up-regulated, whereas STAT3 is down-regulated in the indicated cell lines. Pooled results of n = 6 experiments are shown. b Analysis of STAT3 levels after siRNA-mediated knock-down of CD24. Note that all oligonucleotides specific for CD24 reduce STAT3 expression levels. c Western-blot analysis for STAT3 and p-STAT3 Y705 after siCD24-mediated depletion of CD24. d Decreased STAT3 activity after CD24 or STAT3 knock-down (as a positive control) was measured using a STAT3-specific luciferase reporter assay. Pooled results (b) and representative results (c, d) of n = 4 experiments are shown