Fig. 4.

Loss of CD24 affects transcription of classical STAT3 target genes. a Known STAT3 target genes (cyclin D1, MCL-1, survivin) were analyzed by qPCR after CD24 or STAT3 knock-down in indicated tumor cell lines. Relative mRNA levels normalized to β-actin were compared to siGFP control. b Regulation of the CD24-dependent target gene signature expression after STAT3 silencing was determined by qPCR. Relative mRNA levels normalized to β-actin were compared to siGFP control. Pooled results of n = 4 experiments are shown (a, b)