Fig. 12.

Disorder propensity and structural features of the HIV-1 reverse transciptase. a Crystal structure of the p51-p66 heterodimer (1DLO). b Crystal structure of the p51 bound form, which was computationally extracted from the p51-p66 heterodimer structure (1DLO). c Disorder predisposition of the p66 subunit evaluated by PONDR^® VSL2 (top panel) and PONDR^® VLXT (bottom panel). Red line represents an averaged disorder score for p66 from ~50 different HIV-1 isolates. Pink shadow covers the distribution of disorder scores calculated for p66 from these isolates. Locations of β-strands and α-helices seen in the crystal structure are indicated by gray and black bars between the panels with the disorder scores. Gray shaded area corresponds to the p51 subunit. Dark blue line represents predicted α-MoRF. d NMR solution structure of the RNase H domain of the HIV-1 reverse transcriptase (1O1W). Ten representative members of the conformational ensemble are shown by ribbons of different color