Fig. 4.

CeMBL colocalizes with CAG and CUG RNA foci in the nuclei of CAG125 and CUG125 worm muscles. Cy3-labeled (CTG)₁₀ and (CAG)₁₀ probes were first used to detect RNA foci in CAG125 and CUG125 worms, respectively, by FISH. Subsequently, immunofluorescence staining was performed using an anti-CeMBL antibody. FISH (red), immunofluorescence (green), and nuclear staining (DAPI, blue) images are merged in panels on the right. No RNA foci were detected in GFP (bottom panel) and CAG125 worms (data not shown) when Cy3-labeled (CAG)₁₀ probes were used. Representative high magnification images of colocalization are shown in the left upper boxes of merged figures. Schematic figures are shown to indicate the relative locations of the body wall muscle cells (gray area) in the confocal images