Fig. 5a–d.

Reduced expression of deb-1 in CeMbl (RNAi), CUG125, and CAG125 worms. a Semi-quantitative RT-PCR analysis of deb-1 RNA level in control(RNAi) and CeMbl(RNAi) worms. Representative RT-PCR products were run on a 1% agarose gel and are shown in the left panel. At least three independent experiments were performed, and the relative expression level of the examined RNA is shown in the right panel. Elr-1 is a neuron-specific gene in C. elegans. b Western blot analysis of the DEB-1 protein levels in control(RNAi) and CeMbl(RNAi) worms. The comparison of the DEB-1 protein levels is indicated in the bottom panel. Actin gene expression was used as the internal standard for normalization. Error bars represent standard errors. *P < 0.01. c Semi-quantitative RT-PCR analysis of the deb-1 RNA level in GFP, CAG125, and CUG125 worms. Comparison of the deb-1 RNA level was performed by real-time RT-PCR using primer sequences that are common for all deb-1 isoforms and is shown at the bottom. This experiment was repeated three times using RNA extracted from three independently produced transgenic lines. d Western blot assay of DEB-1 protein levels in GFP, CAG125, and CUG125 worms. Actin served as an internal control for normalization. Error bars represent standard errors. *P < 0.01