Fig. 4.

Effect of NBC and dNBC on cell viability. a Cells were treated for 24 h with increasing concentration of the indicated compounds. Cell viability was assessed by the MTT method, assigning 100% value to the control cells, treated with the solvent. Reported values represent the means ± SEM from four separate experiments. b Cells were treated with 10 μM NBC for 4 h. Apoptosis and necrosis were evaluated by quantification of nucleosomes present in the cytosol (apoptotic cells) and in extracellular medium (released by necrotic cells), using the Cell Detection Elisa kit (Roche). Treatment with 50 μM etoposide was used as a positive control for apoptosis induction. Reported values represent the means ± SEM from two separate experiments. For details, see text