Fig. 1.

a Expression of APC markers by activated Vγ9/Vδ2 T cells, as analyzed by real-time PCR. Purified γδ T cells were co-cultured with EBV-transformed feeder cells in the presence of 100 nM HMB-PP and 100 U/ml IL-2 for up to 42 h. Quantitative RT-PCR was performed with RNA from MACS beads purified γδ T cells and specific primers corresponding to indicated proteins; β-actin and TNF-α served as internal controls. mRNA expression levels shown are mean ± SEM from up to four individual donors, relative to the house-keeping gene cyclophilin. b Uptake and sorting of soluble protein in γδ T-APCs. γδ T-APCs were cultured in the presence of 1 mg/ml FITC-BSA (green) for 1 h. After washing, cells were stained with antibodies to early endosomal markers EEA-1 or CD71 or lysosomal marker lamp-1 (red); nuclei are shown in blue. Confocal images of selected stacks of 15–20 z-planes are shown