Table II.
HPLC determination of mean flavonol composition ± sd of 5-d-old Arabidopsis seedlings divided into sections
| Quercetin
|
Kaempferol
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Line | UH | LH | TZ/UR | LR | UH | LH | TZ/UR | LR |
| ng/mg fresh wt | ng/mg fresh wt | |||||||
| Ler | 26.5 ± 4.2 | 5.3 ± 0.3 | 111 ± 7.9 | 0 ± 0.0 | 42 ± 3.4 | 0 ± 0.0 | 174 ± 10.3 | 84.8 ± 37.3 |
| tt3 | 137 ± 6.2* | 15 ± 0.8* | 275 ± 12* | 10.6 ± 7.22 | 42 ± 4.2 | 1.1 ± 0.4* | 185 ± 16.3 | 127 ± 39.8 |
| tt4 | 0 ± 0.0* | 0 ± 0.0* | 0 ± 0.0* | 0 ± 0.0 | 0 ± 0.0* | 0 ± 0.0 | 0 ± 0.0* | 0 ± 0.0 |
| tt5 | 0 ± 0.0* | 0 ± 0.0* | 0 ± 0.0* | 0 ± 0.0 | 0 ± 0.0* | 0 ± 0.0 | 0 ± 0.0* | 0 ± 0.0 |
| tt7 | 0 ± 0.0* | 0 ± 0.0 | 0 ± 0.0* | 0 ± 0.0 | 63 ± 4.4* | 0 ± 0.0 | 296 ± 14.7* | 111 ± 32.6 |
Results were confirmed with fluorimetry, UV/vis spectroscopy, and mass spectroscopy. LH, Lower hypocotyl; TZ, transition zone; UR, upper root; LR, lower root. Means ± sd, n = 3. Asterisk indicates P < 0.001 compared with Ler by Student-Neumann-Keuls post hoc.