Table 1.
Phosphorylated protein kinases and phosphatases in gastric cancer
| Protein/gene | Protein kinase/phosphatase family | Description | Phosphorylation site | Identified by | Relative expression level in gastric cancer transcriptome | If annotateda | If documented in gastric cancerb | If documented in other cancersc |
|---|---|---|---|---|---|---|---|---|
| Protein kinase | ||||||||
| AAK1/AP2 | Other | AP2 associated kinase 1; adaptor-associated kinase 1 | S623 | MS | 0.97 | Yes | No | Yes |
| AAK1/AP2 | Other | AP2 associated kinase 1; adaptor-associated kinase 1 | S624 | MS | 0.97 | Yes | No | Yes |
| AAK1/AP2 | Other | AP2 associated kinase 1; adaptor-associated kinase 1 | T620 | MS | 0.97 | Yes | No | Yes |
| AKT/AKT1 | AGC | AKT1 kinase | S473 | Antibody | 2.30 | Yes | Yes | Yes |
| AKT/AKT1 | AGC | AKT1 kinase | T308 | Antibody | 2.30 | Yes | Yes | Yes |
| AMPKa1/PRKAA1 | CAMK | Protein kinase, AMP-activated, alpha 1 catalytic subunit | T174 | Antibody | 1.03 | Yes | No | Yes |
| AMPKa2/PRKAA2 | CAMK | Protein kinase, AMP-activated, alpha 2 catalytic subunit | T172 | Antibody | 1.39 | Yes | No | Yes |
| CASK | CAMK | Calcium/calmodulin-dependent serine protein kinase (MAGUK family) | S192 | MS | 1.82 | No | No | Yes |
| CDK3 | CMGC | Cyclin-dependent kinase 3 | T42 | MS | 1.65 | No | Yes | Yes |
| CDK3 | CMGC | Cyclin-dependent kinase 3 | Y43 | MS | 1.65 | No | Yes | Yes |
| DYRK1B | CMGC | Dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B | Y273 | MS | 2.32 | Yes | No | Yes |
| EGFR | TK | Epidermal growth factor receptor | T648 | MS | 2.20 | No | No | No |
| EGFR | TK | Epidermal growth factor receptor | S695 | MS | 2.20 | Yes | No | No |
| EGFR | TK | Epidermal growth factor receptor | T693 | MS | 2.20 | Yes | No | No |
| ERK1/MAPK3 | CMGC | Mitogen-activated protein kinase 3 | T185/Y187d | Antibody | 0.79 | Yes | Yes | Yes |
| ERK1/MAPK3 | CMGC | Mitogen-activated protein kinase 3 | T202/Y204d | Antibody | 0.79 | Yes | Yes | Yes |
| ERK2/MAPK1 | CMGC | Mitogen-activated protein kinase 1 | T185/Y187d | Antibody | 1.46 | Yes | Yes | Yes |
| ERK2/MAPK1 | CMGC | Mitogen-activated protein kinase 1 | T202/Y204d | Antibody | 1.46 | Yes | Yes | Yes |
| FAK/PTK2 | TK | Protein tyrosine kinase 2 | Y397 | Antibody | 1.39 | Yes | Yes | Yes |
| FGR | TK | Proto-oncogene tyrosine-protein kinase FGR | Y412 | Antibody | 0.18 | Yes | No | Yes |
| FYN | TK | Proto-oncogene tyrosine-protein kinase fyn | Y420 | Antibody | 0.34 | Yes | Yes | Yes |
| GSK3A | CMGC | Glycogen synthase kinase 3 alpha | S21/S9d | Antibody | 1.52 | Yes | No | Yes |
| GSK3B | CMGC | Glycogen synthase kinase 3 beta | S21/S9d | Antibody | 1.48 | Yes | No | Yes |
| GSK3B | CMGC | Glycogen synthase kinase 3 beta | Y216 | MS | 1.48 | Yes | No | Yes |
| HCK | TK | Tyrosine-protein kinase HCK | Y411 | Antibody | 0.26 | Yes | Yes | Yes |
| INSR | TK | Insulin receptor; CD220 | S720 | MS | 0.62 | No | No | Yes |
| JNK1/MAPK8 | CMGC | Mitogen-activated protein kinase 8 | T183/Y185d | Antibody | 1.28 | Yes | Yes | Yes |
| JNK1/MAPK8 | CMGC | Mitogen-activated protein kinase 8 | T221/Y223d | Antibody | 1.28 | Yes | Yes | Yes |
| LCK | TK | Lymphocyte-specific protein tyrosine kinase | Y394 | Antibody | 0.23 | Yes | Yes | Yes |
| LYN | TK | Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog | Y397 | Antibody | 0.79 | Yes | Yes | Yes |
| MARK2 | CAMK | MAP/microtubule affinity-regulating kinase 2 | S423 | MS | 1.28 | Yes | Yes | Yes |
| MEK1/MAP2K1 | STE | Mitogen-activated protein kinase kinase 1 | S218/S222d | Antibody | 1.64 | Yes | Yes | Yes |
| MEK1/MAP2K1 | STE | Mitogen-activated protein kinase kinase 1 | S222/S226d | Antibody | 1.64 | Yes | Yes | Yes |
| MEK2/MAP2K2 | STE | Mitogen-activated protein kinase kinase 2 | S218/S222d | Antibody | 1.64 | Yes | Yes | Yes |
| MEK2/MAP2K2 | STE | Mitogen-activated protein kinase kinase 2 | S222/S226d | Antibody | 1.64 | Yes | Yes | Yes |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | S1006 | MS | 4.33 | No | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | S1008 | MS | 4.33 | No | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | T1011 | MS | 4.33 | No | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | T678 | MS | 4.33 | No | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | T992 | MS | 4.33 | No | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | T993 | MS | 4.33 | No | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | Y666 | MS | 4.33 | No | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | S1000 | MS | 4.33 | Yes | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | S988 | MS | 4.33 | Yes | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | S990 | MS | 4.33 | Yes | No | No |
| MET | TK | Met proto-oncogene (hepatocyte growth factor receptor) | S997 | MS | 4.33 | Yes | No | No |
| MSK1/RPS6KA5 | AGC | Ribosomal protein S6 kinase, 90 kDa, polypeptide 5 | S376/S360d | Antibody | 0.29 | Yes | No | Yes |
| MSK2/RPS6KA4 | AGC | Ribosomal protein S6 kinase, 90 kDa, polypeptide 4 | S376/S360d | Antibody | 2.27 | Yes | No | Yes |
| MTOR/FRAP1 | Atypical | Mechanistic target of rapamycin (serine/threonine kinase) | S2448 | Antibody | 1.25 | Yes | Yes | Yes |
| P38a/MAPK14 | STE | Mitogen-activated protein kinase 14 | T180/Y192d | Antibody | 2.37 | Yes | No | Yes |
| P70S6K/RPS6KB1 | AGC | Ribosomal protein S6 kinase, 70 kDa, polypeptide 1 | T229 | Antibody | 1.02 | Yes | Yes | Yes |
| p70S6K/RPS6KB1 | AGC | Ribosomal protein S6 kinase, 70 kDa, polypeptide 1 | T389 | Antibody | 1.02 | Yes | Yes | Yes |
| p70S6K/RPS6KB1 | AGC | Ribosomal protein S6 kinase, 70 kDa, polypeptide 1 | T421/S424d | Antibody | 1.02 | Yes | Yes | Yes |
| PKD2 | CAMK | Polycystic kidney disease 2 (autosomal dominant) | S812 | MS | 0.55 | Yes | Yes | Yes |
| PKN2 | AGC | Polycystic kidney disease 2 (autosomal dominant) | S582 | MS | 1.70 | Yes | No | No |
| PKN2 | AGC | Polycystic kidney disease 2 (autosomal dominant) | S583 | MS | 1.70 | Yes | No | No |
| PYK2/PTK2B | TK | Protein tyrosine kinase 2 beta | Y402 | Antibody | 1.13 | Yes | Yes | Yes |
| RSK1/RPS6KA1 | AGC | Ribosomal protein S6 kinase, 90 kDa, polypeptide 1 | S221 | Antibody | 1.06 | Yes | No | Yes |
| RSK1/RPS6KA1 | AGC | Ribosomal protein S6 kinase, 90 kDa, polypeptide 1 | S380 | Antibody | 1.06 | Yes | No | Yes |
| RSK2/RPS6KA3 | AGC | Ribosomal protein S6 kinase, 90 kDa, polypeptide 3 | S221 | Antibody | 2.46 | Yes | No | Yes |
| RSK2/RPS6KA3 | AGC | Ribosomal protein S6 kinase, 90 kDa, polypeptide 3 | S380 | Antibody | 2.46 | Yes | No | Yes |
| RSK3/RPS6KA2 | AGC | Ribosomal protein S6 kinase, 90 kDa, polypeptide 2 | S380 | Antibody | 0.39 | Yes | No | Yes |
| SMG1 | Atypical | Phosphatidylinositol 3-kinase-related protein kinase | S2940 | MS | 0.93 | No | No | Yes |
| SMG1 | Atypical | Phosphatidylinositol 3-kinase-related protein kinase | S2946 | MS | 0.93 | No | No | Yes |
| SMG1 | Atypical | Phosphatidylinositol 3-kinase-related protein kinase | T2947 | MS | 0.93 | No | No | Yes |
| SRC | TK | Proto-oncogene tyrosine-protein kinase | Y419 | Antibody | 1.11 | Yes | Yes | Yes |
| SRPK2 | CMGC | Serine/arginine-rich protein-specific kinase 2 | S496 | MS | 1.53 | No | No | Yes |
| SRPK2 | CMGC | Serine/arginine-rich protein-specific kinase 2 | T492 | MS | 1.53 | No | No | Yes |
| SRPK2 | CMGC | Serine/arginine-rich protein-specific kinase 2 | S494 | MS | 1.53 | Yes | No | Yes |
| SRPK2 | CMGC | Serine/arginine-rich protein-specific kinase 2 | S497 | MS | 1.53 | Yes | No | Yes |
| SRPK2 | CMGC | Serine/arginine-rich protein-specific kinase 2 | T498 | MS | 1.53 | Yes | No | Yes |
| TNIK | STE | TRAF2 and NCK interacting kinase | S680 | MS | 1.25 | Yes | No | Yes |
| TTBK2 | CK1 | Tau tubulin kinase 2 | T1070 | MS | 0.75 | No | No | No |
| YES/YES1 | TK | Yamaguchi sarcoma viral oncogene homolog 1 | Y426 | Antibody | 0.86 | Yes | Yes | Yes |
| Protein phosphatase | ||||||||
| PTPN14 | PTP | Tyrosine-protein phosphatase non-receptor-type 14 | S312 | MS | 3.58 | No | No | Yes |
| PTPN14 | PTP | Tyrosine-protein phosphatase non-receptor-type 14 | S314 | MS | 3.58 | No | No | Yes |
| PTPRF | PTP | Isoform 1 of receptor-type tyrosine-protein phosphatase F | T1801 | MS | 3.26 | No | No | Yes |
| PTPRF | PTP | Isoform 1 of receptor-type tyrosine-protein phosphatase F | T1811 | MS | 3.26 | No | No | Yes |
| PTPRF | PTP | Isoform 1 of receptor-type tyrosine-protein phosphatase F | T1825 | MS | 3.26 | No | No | Yes |
| PTPN12 | PTP | Tyrosine-protein phosphatase non-receptor-type 12 | S435 | MS | 2.89 | Yes | No | Yes |
| PTPRA | PTP | cDNA FLJ56484, highly similar to receptor-type tyrosine-protein phosphatase alpha | S171 | MS | 2.89 | No | Yes | Yes |
| PTPRA | PTP | cDNA FLJ56484, highly similar to receptor-type tyrosine-protein phosphatase alpha | S172 | MS | 2.89 | No | Yes | Yes |
| PTPRA | PTP | cDNA FLJ56484, highly similar to receptor-type tyrosine-protein phosphatase alpha | T161 | MS | 2.89 | No | Yes | Yes |
| MTMR7 | DSP | Isoform 1 of myotubularin-related protein 7 | S213 | MS | 0.86 | No | No | No |
List of protein kinases and phosphatases identified by LC-MS/MS-based and protein antibody array-based phosphoproteomics analysis of gastric cancer cell lines and primary gastric tissues. The expression of each protein kinase and phosphatase gene in the transcriptomes of 17 gastric cancer cell lines relative to normal stomach tissues is shown. The values are the average of 17 gastric cancer cell lines
aThe phosphorylation site was annotated previously in the literature
bThe protein’s role in gastric cancer was documented previously in the literature
cThe protein’s role in other cancers was documented previously in the literature
dThe antibody detects both phosphorylation sites in the protein kinase