Fig. 10.
Amyloidosis and inflammatory response in young mice. a Representative WB band pattern obtained with WO2 antibody in prefrontal cortex homogenates from 5-month-old mice. b WB analysis showed no differences between genotypes in the APP content but decreased levels of CTF-β in MT1 + 2 null mice from both genders, perhaps suggesting a decreased β-secretase activity. Although by WB the monomeric forms of Aβ could not be detected, ELISA analysis (c) revealed that at 5 months of age, Aβ1–40 but not Aβ1–42 levels were already increased in APP+ compared to APP− mice, although no differences due to the absence of MT1 + 2 could be observed at that age. d RNase protection assay (RPA) of prefrontal cortex mRNA hybridized with a set of host response genes. e Quantification of these hybrids in males showed that both hAPP presence and MT1 + 2 absence altered the mRNA levels at this early age, suggestive of an increased inflammatory response. Data represents mean ± SEM (n = 4–6) and was analyzed using GLZ. Plus, p < 0.05 versus APPWT mice; filled diamond, p ≤ 0.001 versus APP− mice; star, p < 0.05 versus MTWT mice; spade, p < 0.05 significant interaction between both factors