Fig. 7.

Increased CTF-β and Aβ trimeric forms in the cortex of MT1 + 2 null males. a, b Representative WB band pattern obtained with 6E10 antibody in total cortical homogenates and fraction C (membrane-associated protein enriched fraction), respectively, of male mice. Apart from the specific bands mentioned in Fig. 6, a 14-kDa band, that by its size could represent a trimer of Aβ, was also quantified. MT1 + 2 deficiency in APP+ male mice significantly increased CTF-β and putative Aβ trimer levels in total cortical homogenates (c), and Aβ trimer levels in Fraction C (d). Analysis of female mice total cortical homogenates (e) showed no differences between APP+ genotypes, while in the extracellular protein enriched fraction (f) the 14-kDa band was significantly decreased by MT1 + 2 deficiency. Data represents mean ± SEM (n = 8–13). Plus, p at least ≤0.03 versus APPWT mice