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The HINT1 protein forms a homodimer in living cells. Immunodetection of HINT1 in the synaptosome-rich preparation obtained from the cerebral cortex and PAG of HINT1+/+ and HINT1−/− SvJ mice. The HINT1 monomer and homodimer are indicated. KO: HINT1−/− SvJ mice. Visualization of the HINT1 homodimer in BiFC assays on CHO cells. The confocal fluorescent signals are obtained when a molecule of HINT1VN173 and HINT1VC155 associate. The arrows indicate positive cells in the field
