Fig. 6.

The RGSZ2.Gαi2/z complex associates with dephosphorylated HINT1 proteins. a Gαi2 and Gαz subunits (100 nM) display no affinity toward the HINT1 protein (200 nM). b RGSZ2 (100 nM) and GαGTP subunits (100 nM) were incubated with HINT1 (200 nM) protein and after precipitating the Gα, the HINT1 homodimer associated to RGSZ2 was evaluated. IP immunoprecipitation. Proteins were used without tags and they were immunoprecipitated with affinity-purified antibodies against the corresponding antigenic peptide. c Selectivity of the antibodies for the recombinant proteins used