Fig. 7.

Antimicrobial activities of recombinant β-defensins. a–c Dose effects of recombinant β-defensins on antimicrobial activities. E. coli K12D31 (a), S. aureus CMCC26003 (b) and C. albicans sc5314 (c) were incubated in 10 mM phosphate buffer (pH 7.4) in the presence of recombinant peptides for 2 h (E. coli and S. aureus at 37°C; C. albicans at 30°C). Incubation with the same volume of elution buffer was used as control in the antimicrobial tests. d Antimicrobial activities of Defb15 at various pH values (pH 6.0, 6.5, 7.0 and 7.5). Incubation with the elution buffer of recombinant Defb15 with the same volume and pH value was used as control. The number of CFU was determined by colony counts after serial dilutions. The experiments were independently replicated four times. Values are mean ± SEM