Fig. 4.

Gene expression pattern in human mast cells following IgE sensitization and FcεRI aggregation. The raw Affymetrix GeneChip data was downloaded from gene expression omnibus (GSE1933) and normalized with parametric test based on cross gene error model (PCGEM) and subjected to one-way ANOVA and Bonferroni-Hochberg FDR (p < 0.05) using Genespring 7.3 The differentially expressed genes by the IgE sensitization and FcεRI cross-linking for different time points (2, 6, and 12 h) were then classified and clustered based on gene ontology (GO). Analysis to decode the differentially expressed genes implicated in biological processes such as a cytokines and cytokine receptors, b chemokines and chemokine receptors, c other immunoregulatory genes, d cell proliferation and apoptosis, e adhesion and cytoskeleton remodeling, f transcription factors and regulators, g signal transduction, and h other genes [115]