Fig. 1.
PrPC and Shadoo, but not Doppel, affect HIV-1 expression and production. a Schematic representation of the PrPC, Doppel and Shadoo GPI anchor proteins. b Viral supernatants from HIV-1/Control (CT)/PLAP (placental alkaline phosphatase)/human huPrPC and /huPrPCΔGPI or /murine moPrPC,/moDoppel and/moShadoo co-transfected cells were harvested 48 h after transfection and the virus-associated reverse transcriptase (RT) activity measured. Data shown are representative of three independent experiments. Values are given as means ± SD. c Virus-producer cells were analyzed by Western blotting. Total protein (10 μg per lane) of HIV-1/CT (lane 1); HIV-1/PLAP (lane 2); HIV-1/WT-PrPC (lane 3); HIV-1/PrPCΔGPI (lane 4); HIV-1/moPrPC (lane 5); HIV-1/moDoppel (lane 6); HIV-1/moShadoo (lane 7) was analyzed by SDS PAGE. Membranes were probed with antibodies directed against HIV-1 CAp24, PLAP, PrPC, Flag tag for PrPC and moDoppel, moShadoo. Cyclophilin A (CypA) and coomassie staining were used as loading controls