Fig. 2.
Anti-viral properties of PrPC map to the N-terminal region. a Schematic representation of PrPC with amino acid positions and deleted domains. b Viral supernatants from 293T cells co-transfected with HIV-1 pNL43 and control vector (CT), full length WT PrPC (WT), or PrPC deletion mutants PrP-ΔGPI, Δ24-145, Δ24-28, Δ36-50, ΔOR, Δ95-110 or ΔHC were harvested 48 h after transfection and released virus associated RT activity was measured. Data shown are representative of six independent experiments. Values are given as means ± SD. c Virus producer cells were analyzed by Western blotting. Total protein (10 μg per lane) of HIV-1/CT (lane 1); HIV-1/PrPC-WT (lane 2); HIV-1/PrP-ΔGPI (lane 3); HIV-1/PrP-ΔHC (lane 4); HIV-1/PrP-Δ24-28 (lane 5); HIV-1/PrP-Δ24-145 (lane 6) was analyzed by SDS PAGE. Membranes were probed with antibodies directed against HIV-1 CAp24 and PrPC. Cyclophilin A (CypA) and Coomassie staining were used as loading controls. Note that the Pr55Gag protein level was strongly reduced in PrPC-WT and ΔHC samples