Fig. 4.
PrPC is expressed in HIV-1 target cells. a Western blotting analysis of PrPC expressing cells; 10 μg of total protein extracts of cell lines or peripheral blood mononuclear cells was analyzed by SDS-PAGE, and transferred membranes were probed with the monoclonal SAF32 anti-PrP. Lane 1 lymphoblastoid cell line CEM-GFP; lane 2 monocytes; lane 3 monocyte-derived dendritic cells (MDDC); lane 4 activated peripheral blood lymphocytes (PBLs); lane 5 monocyte-derived macrophages (MDM); lane 6 the glioblastoma cell line U251MG. b Distribution of PrPC by confocal immunofluorescence in U251MG cells (panel 1); CEM lymphoblastoid cells (panel 2); primary monocytes (panel 3); monocyte-derived dendritic cells (MDDC, panel 4); PBLs, panel 5; monocyte-derived macrophages (MDM, panel 6). Note the presence of PrPC signal (green) at the plasma membrane, in filopodia (see white arrows for U251MG cells), in tunneling nanotubes (see white arrowheads in CEM and MDDC) and at the immunological synapse (see double white arrowheads for activated PBL). Scale bars are 10 μm