Fig. 7.

Certain long HIF-3α variants upregulate HIF target genes when HIF-β is not limiting. Hep3B cells were transfected with a plasmid encoding the full-length long HIF-3α variants with (a) or without (b) HIF-β. HIF-3α4 was excluded here as the aim was to study the transactivation potential of HIF-3α variants. The cells were cultured for 48 h in normoxia followed by 90 min in 1% oxygen (a) or for 24 h in normoxia followed by 24 h in 1% oxygen (b). The expression levels of the EPO, ANGPTL4, GLUT1, PFKL, HO-1 and VEGF mRNAs were analyzed by qPCR. The data represent means ± SD from at least three independent experiments. *p < 0.05, **p < 0.01,***p < 0.001