Table 1.
Sequences of the oligos used in RNA interference and qPCR analyses of the HIF-3α variants
| Primer | Sequences (5′- 3′) and locations of the oligos | Use |
|---|---|---|
| 3α_a (D-010068-02) | GCAAGAGCAUCCACACCUU, exon 7 and 8 | RNA interference |
| 3α_b (D-010068-05) | CGACAGGAUUGCAGAAGUG, exon 6 and 7 | RNA interference |
| 3α_c (D-010068-21) | UAACAGGGCAGUAUCGCUU, exon 8 | RNA interference |
| TBP_F | GAARARAARCCCAAGCGGTTTG | qPCR |
| TBP_R | ACTTCACATCACAGCTCCCC | qPCR |
| HIF1a_Q_F | CTAGCTTTGCAGAATGCTCAG | qPCR |
| HIF1a_Q_R | GTAGTAGCTGCATGATCGTCTG | qPCR |
| HIF2a_Q_F | CCCAGATCCACCATTACAT | qPCR |
| HIF2a_Q_R | ACTCCAGCTGTCGCTTCA | qPCR |
| Epo_F | CTCCGAACAATCACTGCT | qPCR |
| Epo_R | GGTCATCTGTCCCCTGTCCT | qPCR |
| LOX_QF | ACGGCACTGGCTACTTCCAG | qPCR |
| LOX_QR | GGCCAGACAGTTTTCCTCCG | qPCR |
| Q_GLUT_F | GATTGGCTCCTTCTCTGTGG | qPCR |
| Q_GLUT_R | TCAAAGGACTTGCCCAGTTT | qPCR |
| Q_HK2_F | CAAAGTGACAGTGGGTGTGG | qPCR |
| Q_HK2_R | GCCAGGTCCTTCACTGTCTC | qPCR |
| Q_PFKL_F | CCCGAGGACGGCTGGGAGAA | qPCR |
| Q_PFKL_R | GGGCTTCCCGTTGCGGTCAA | qPCR |
| Q_TRF_F | GTCGCTGGTCAGTTCGTGATT | qPCR |
| Q_TRF_R | AGCAGTTGGCTGTTGTAACCTCTC | qPCR |
| Q_uPAR_F | GGTGACGCCTTCAGCATGA | qPCR |
| Q_uPAR_R | CCCACTGCGGTACTGGACAT | qPCR |
| Q_BNIP_F | GGACAGAGTAGTTCCAGAGGCAGTTC | qPCR |
| Q_BNIP_R | GGTGTGCATTTCCACATCAAACAT | qPCR |
| Q_AngPT4_F | TCTCCGTACCCTTCTCCACT | qPCR |
| Q_AngPT4_R | AGTACTGGCCGTTGAGGTTG | qPCR |
| Q_HO1_F | CTATGTGAAGCGGCTCCACG | qPCR |
| Q_HO1_R | GCTCTGGTCCTTGGTGTCAT | qPCR |
| Q_VEGF_F | AGCCTTGCCTTGCTGCTCTA | qPCR |
| Q_VEGF_R | TCAGGGTACTCCTGGAAGATG | qPCR |