Fig. 1.

Expression of Nogo receptor complex elements and effects of myelin in TEG3 cell adhesion. a–d Photomicrographs illustrating the expression of p75 (a), NgR1 (b), GFAP (c), and S100β (d) in cultured TEG3 cells. e Western blotting detected the expression of p75, NgR1, Lingo 1, and TROY in cultured TEG3 cells and brain extracts (see the section Materials and methods for details). Actin was blotted as internal control. f Photomicrographs illustrating an example of the distribution of TEG3 cells cultured on glass substrates containing spots of Alexa-tagged myelin. Note that TEG3 cells (Phalloidin-positive, red) are not localized on myelin containing regions (green). g, h Examples of stripe assays using TEG3 cells. In both cases, a coating of poly-l-lysine was uniformly distributed in all the culture area. Alternative stripes containing Alexa594-tagged BSA + laminin and laminin were generated (g) or Alexa594-tagged BSA + laminin and myelin (h). DAPI-stained TEG3 cells do not adhere in myelin-containing stripes (h) (see also Results). Scale bars: a = 25 μm pertains to b–d; f = 50 μm; g and h = 50 μm