Fig. 2.

Expression profile of Hsp60 in leucocytes and erythrocytes. a Serum-starved leucocytes and monocytes were stained with BOB78 and IgM isotype control antibodies, with secondary detection by anti-mouse FITC-conjugated antibodies. Surface expression was analysed by flow cytometry. Cells were gated by size (forward scatter, FS) into dying cells (A) and viable cells (V). b Apoptotic leucocytes expressed Hsp60 on the surface. c Single peak of Hsp60 detection in permeabilized leucocytes, indicating the presence of intracellular Hsp60 in apoptotic and viable cells. d Erythrocytes were fixed, permeabilized, and stained with BOB78 and glycophorin A (control positive) antibodies, with secondary detection by anti-mouse FITC-conjugated antibodies. e Intracellular glycophorin was detectable in erythrocytes. f Hsp60 was not detected in erythrocytes