Fig. 6.

Differentiated fetal NS cells produce action potentials and exhibit ligand-gated channel currents. a Sample records of the total current derived from the NS12SC cell demonstrate the presence of a tetrodotoxin-sensitive Na⁺ current (right). A representative overshooting action potential, evoked on the same cell, is shown on the left. b RT-PCR performed on NS12ST cells in proliferation (P) and in differentiation for the expression of the GABA_B receptor-coding gene (Gabbr1). c Sample traces showing the effects of GABAergic agonists on differentiated NS12ST cells. Coapplication of dopamine (100 μM) significantly reduced the inward current evoked by GABA (20 μM) in differentiated NS12ST cells. This effect was partially reversible. d RT-PCR performed on NS12SC cells in proliferation (P) and in differentiation (D). e Sample trace showing the current evoked by GABAergic agonist on differentiated NS12SC cells