Table 1.
RT-PCR primer sequences, related conditions and amplicons
| Gene | Forward primer | Reverse primer | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
| Arpp21 | ATGTCTGAGCAAGGAGGACTG | TCTCTTGGTTCTGAGCCGC | 58 | 154 |
| Ascl1 | CGTCCTCTCCGGAACTGAT | CGTCCTCTCCGGAACTGAT | 56 | 482 |
| Ascl1 (RT-qPCR) | CACGAGAAGAGTGACTGGTGT | GGCTAAGACAGCTCTGACAGA | 58 | 194 |
| β-Actin | GGCCCAGAGCAAGAGAGGTATCC | ACGCACGATTTCCCTCTCAGC | 58 | 460 |
| β-Actin (RT-qPCR) | AGTGTGACGTTGACATCCGTA | GCCAGAGCAGTAATCTCCTTCT | 60 | 112 |
| Blbp | GGGTAAGACCCGAGTTCCTC | ATCACCACTTTGCCACCTTC | 56 | 213 |
| Chrm4 | TCCTCACCTGGACACCCTAC | ACGTAGCAGAGCCAGTAGCC | 60 | 103 |
| Dlx5 | AGCCCCTACCACCAGTACG | TGTGTTTGCGTCAGTCCTAGAGAG | 58 | 245 |
| Dlx6 | TCAATACCTGGCCCTTCCCG | GGTGTCCTGGTGTGGTGAG | 58 | 292 |
| Drd1 | GAGCGTAGTCTCCCAGATCG | GGATGCTGCCTCTTCTTCTG | 58 | 104 |
| Drd2 | CAAGCGTAGCAGCCGAGCTT | CATTCTCCGCCTGTTCACTG | 60 | 136 |
| Emx2 | ACGCTTTTGAGAAGAACCATTACGT | GTCTGAGGTCACATCTATTTCCTCC | 60 | 242 |
| Foxg1 | CTGAGTGTGGACCGGCTG | CGTGCTGGTCTGCGAAGTC | 58 | 213 |
| Foxp1 | TGACAAGCAACCAGCTCTTCAG | ATGTTGCTGTTGGAGAAGTTGAAGC | 58 | 226 |
| Foxp2 | TCACTTATGCAACACTCATAAGGCAG | CGCTTCTGGTATTCGACTTCGTC | 58 | 243 |
| Gabbr1 | AGGTTGATCACTCGAGGTGAA | CATCTGTTTCCCAGAGTACTGA | 56 | 476 |
| Gfap | GCTCCAAGATGAAACCAACCTGAG | CTTAAGACTGGATCTCCTCCTCCA | 56 | 554 |
| Glast | CCAAAAGCAACGGAGAAGAG | ACCTCCCGGTAGCTCATTTT | 56 | 229 |
| Glra1 | GAAGCCTGACTTGTTCTTTGCC | GTTGCATGATACACGTCTGTAC | 58 | 185 |
| Glra2 | CGTACAGTGAGTACCCAGATG | CAACTTGCACTGGACCATCAC | 58 | 315 |
| Gsx2 | CACTACCTACAACATGTCGGACC | CCTTCTTGTGCTTCACGCGAC | 58 | 254 |
| Hb9 | TCGAACCTCTTGGGGAAGTG | AATCTTCACCTGAGTCTCGGT | 61 | 159 |
| Hoxa4 | TGTGCCCTACTCATCTCCTG | GCTGTCTGGCTGACTCAAAG | 53 | 260 |
| Hoxb4 | TGCAAAGAGCCCGTCGTC | GGAACCAGATCTTGATCTGGCG | 60 | 185 |
| Hoxb9 | TCCATTTCTGGGACGCTTAG | CCCTCCTGAGGTACATATTG | 60 | 230 |
| Irx3 | GACGAGGAGGGCAATGCTTAT | AAATGGGTCCCAGGCCGAAGT | 60 | 259 |
| Islet-1 | CGTCTGATTTCCCTGTGTGTTGG | AAGTCGTTCTTGCTGAAGCCTATG | 58 | 239 |
| Lim3 | ACAGCGACGCCGAAGTCT | ACTGCTCTGGACCCGTCAAG | 60 | 168 |
| Meis2 | GAGACGTCTGTTCCTCTGACTC | TCTCATCAATCACGAGGTCAATGG | 58 | 243 |
| Nkx2-1 | ACCGGGTTCAGACTCAGTTC | ATCGACATGATTCGGCGTCGG | 60 | 221 |
| Nkx6-1 | TACTTGGCAGGACCAGAGAG | CGCTGGATTTGTGCTTTTTC | 58 | 270 |
| NMDAR1 | TTGGCCGATTTAAGGTGAAC | AAAACCACATGGCAGAGGAC | 58 | 72 |
| Olig2 | GGCGGTGGCTTCAAGTCATC | TAGTTTCGCGCCAGCAGCAG | 60 | 260 |
| Otx1 | GGCGCTGTTCGCAAAGACTC | AGTGGCTCTGGCACCGATAC | 60 | 429 |
| Otx1 (RT-qPCR) | GTCCAGAGTCCAGGTTTGGTTC | GACGAAGAAGCAGAGCTAGATACG | 58 | 180 |
| Otx2 | TACCTCAGTCCCAACCATTG | TCTGAGAGCATCGTTCCATC | 58 | 572 |
| Pax6 | AGTCACAGCGGAGTGAATCAGC | AGCCAGGTTGCGAAGAACTCTG | 58 | 426 |
| Pax7 | GTCACTAAGCATGGGTAGATG | GCTACCAGTACAGCCAGTATG | 56 | 328 |
| Pax7 (RT-qPCR) | CATGAACCCTGTCAGCAATGG | CTGTAGCCAGTGGTGCTGTA | 58 | 230 |
| Rarβ1 | CTTCTGTGTGATTCTGGACCTGTAG | CCACTGGGAGGTGGGTG | 58 | 219 |
| Shh | TCTGTGATGAACCAGTGGCC | GCCACGGAGTTCTCTGCTTT | 60 | 242 |
| Six3 | CAGCAGAGTCACCGTCCAC | TGGAGGTTACCGAGAGGATCG | 58 | 100 |
| Sox2 endo | TAGAGCTAGACTCCGGGCGATGA | TTGCCTTAAACAAGACCACGAAA | 56 | 297 |
| Tlx | TGTGGTGACCGCAGCTC | TCGACACGCCCTGCATTG | 58 | 168 |
| Vglut2 | TCGGCTTCTGCATATCCTTC | CCTGGAATCTGGGTGATGAT | 56 | 190 |