Fig. 5.

NO donor and cell membrane permeable analogue of cGMP facilitates cell migration. a Representative photomicrographs of hNPCs after 24 h of migration under control condition and in the presence of 10 μM NOC-18 and 100 μM NOC-18. b The migration of cells increased significantly in the presence of 1–10 μM of NOC-18. The viability of cells was not affected by NOC-18 application as determined by (c) CellTiter-Blue assay and (d) percent LDH released. e Co-application of NOC-18 (10 μM) together with down-stream enzyme inhibitor of sGC (ODQ, 50 μM) or PKG (Rp-8-Br-cGMP, 1 μM) reduced NO-induced migration of cells. f 8-Br-cGMP facilitated cell migration. g ROCK inhibitor Y27632 (50 μM) blocks cell migration. Data represent mean ± SEM of at least 15 neurospheres (b, e, f, and g) and four measurements (c and d). Scale bar 500 μm