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Schematic diagram of domain structures of the human MBD1 protein and the EGFP-MBD-NLS probe. a Domain structures of human MBD1. MBD, NLS, three CXXC domains and TRD are represented. b Structure of EGFP-MBD-NLS. MBD and NLS of the full-length human MBD1 protein were fused with EGFP and used as the probe for detecting methylated DNA. c Deduced amino acid sequences of the junction region of EGFP-MBD-NLS
