Fig. 5.

Human PBMCs secrete CCN1. a Intracellular staining for CCN1 of CD3⁺ and CD14⁺ cells treated with BFA. Cells were either unstimulated in medium with 10 % AB serum or stimulated with 100 ng/ml TNF-α, 1 μg/ml EGF or 100 ng/ml MCP-1 for 18 h, respectively. Staining for CCN1 was performed with polyclonal anti-CCN1-Biotin antibody that was detected by streptavidin-PE. As background staining control, streptavidin-PE was used alone. Shown is one representative experiment and a table with results from all donors (n = 7). The difference in percent of CCN1-positive cells was calculated using Wilcoxon signed-rank test. b PBMCs were kept for 5 h either in medium containing 10 % AB serum alone or stimulated with TNF-α in the presence (n = 9) or absence (n = 5) of BFA. Lysates and supernatants of the cells were measured for CCN1 by ELISA. Statistical analysis was performed using the Mann–Whitney U test with *p < 0.05