Fig. 3.

Effect of ankyrin and coiled-coil region of synphilin-1 on the degradation of alpha-synuclein by the 20S proteasome. The panels show representative Coomassie Blue-stained gels of the degradations reactions of alpha-synuclein (Snca) wild-type and its missense mutants by 20S proteasome in the presence of synphilin-1 (Sncaip, amino acids 331–555). Reaction conditions: 69.4 nM proteasome, 7 μM alpha-synuclein, and 0.92 μM synphilin-1 (331–515). Control reactions with synphilin-1 and alpha-synuclein for 60 min in the absence of proteasome are also shown