Fig. 4.

Synphilin-1 inhibits the degradation of alpha-synuclein by interacting with the N-terminal region of alpha-synuclein. a Representative experiments analyzed by SDS-PAGE and Coomassie Blue staining and quantification of the degradation of N-terminal His-tag alpha-synuclein (His-Snca wt, 7 μM) full length by 20S proteasome (69.4 nM) in the absence and in the presence of synphilin-1 (Sncaip 331–919). b Similar experiments and quantification using N-terminal His-tag alpha-synuclein 1–50 (His-Snca 1–60, 7 μM)