Fig. 7.

Effect of synphilin-1 on the degradation of unstable fluorescent proteins. HeLa cells were transfected with EGFPu or EGFPd2 and co-transfected with synphilin-1 (Sncaip), as indicated. Cells 48 h after transfection were treated with cycloheximide (CHX). The panels show representative immunoblots with anti-EGFP antibodies, and anti-tubulin antibodies used as protein loading controls. Quantifications are shown in the right graph as means ± SEM from three different experiments