Fig. 8.

Siah-1 effects on synphilin-1 and alpha-synuclein degradation. HeLa cells were single, double, or triple transfected with different plasmids. a Representative immunoblots of the steady-state expression of synphilin-1 (Sncaip) after transfection in the absence or in the presence of Siah-1, as indicated. The blots have been over-exposed in order to show synphilin-1 expression in the presence of transfected Siah-1. b HeLa cells 48 h after transfection with the indicated plasmids were treated with cycloheximide (CHX). Representative immunoblots with anti-Myc (synphilin-1, Sncaip) and anti-HA (Siah-1) are presented and data quantification are shown in d. c HeLa cells 48 h after transfection with the indicated plasmids were treated with CHX. Representative immunoblots with anti-alpha-synuclein (Snca) antibodies are presented and data quantification are shown in d. d Graph showing the quantification of data presented in b and c expressed as means ± SEM from three different experiments. Anti-tubulin antibodies were used as protein-loading controls