Fig. 2.

Relative astakine 1 and astakine 2 mRNA expression show circadian rhythmicity. a Time course in relative expression levels of astakine 1 and astakine 2 in hemocytes (HC) at clock hour 2:00, 6:00, 7:30, 8:00, 12:00, 15:00, 16:30, and 21:00 (n = 6). b Time course in relative expression levels of astakine 1 and astakine 2 in HPT at clock hour 2:00, 6:00, 7:30, 8:00, 12:00, 15:00, 16:30, and 21:00 (n = 6). Asterisks indicate that the expression levels are significantly different (*p < 0.05, **p < 0.01). Light (open square) and dark (filled square) periods in the cycle are indicated on the top of the graph. Error bars indicate standard error of the mean (SEM)