Figure 5.

Reduced anthocyanin accumulation in low GSH biochemical mutants. Low GSH antisense lines (R17-1, R17-2, R17-3, and R12-1), high GSH sense line (16-A), and wild-type plants were germinated and grown side by side under continuous light of 50 μE m⁻²s⁻¹ at 22°C and well watered in a growth chamber for 5 weeks. No apparent phenotypic differences other than size were observed between low GSH plants and wild-type plants under these conditions. The plants were transferred to continuous light of 250 μE m⁻² s⁻¹ at 22°C without watering for 1 week. Anthocyanin accumulation becomes apparent in the leaves of wild-type plants (right in A) but not in low GSH antisense line R17-1 (left in A). Anthocyanin extracts of these lines are shown in B. Concentrations of anthocyanin (C) and GSH (D) were determined as described in “Materials and Methods.” The results shown are means for three to five plants.