Fig. 1.

Aspirin down-regulates cell surface EGFR and increases total protein expression of EGFR and TfnR. A-431 was treated with indicated concentrations of aspirin for 48 h. a Flow cytometry was performed to assess levels of cell surface EGFR. Histogram, Gmean is representative of two independent experiments. b Cell lysates were subjected to WB and total EGFR and TfnR expressions were normalized to untreated sample. Columns average of normalized values from three experiments; bars ± SD. Results with significant statistical difference from untreated sample: *p < 0.05 and **p < 0.001. c mRNA transcription was analyzed by RT-PCR