Fig. 7.

The acetylating property of aspirin is not required for the aspirin-mediated intracellular accumulation of TfnR in the ESE. Indirect immunofluorescence at kinetics was carried out for TfR in A-431 cells. Cells were incubated with anti-TfnR for 1 h on ice. Thereafter, receptor–antibody complexes were allowed to be endocytosed in the presence of indicated concentrations of salicylic acid (SA) for 2 h. Cells were fixed and then co-stained with anti-EEA-1 primary antibody, followed by secondary antibodies. Image of confocal microscope, scale bars 25 μm