Fig. 10.

Cells depleted of TACC are hypersensitive against thiabendazole. Cell types indicated on top were treated with 100 μM thiabendazole for 3 h and microtubules were labeled with anti-tubulin (e–h, green in a, b and red in c, d). Thiabendazole treatment clearly has an effect on all cell types, but compared to wild-type cells remaining microtubules in TACC-RNAi cells are significantly shorter. Expression of GFP-TACCdom (green in c) rescues the hypersensitivity phenotype, while overexpression of GFP-CP224 (green in d) has no significant effect. In merged images DNA is shown in blue. Bar 2 μm