Fig. 11.

CP224 localization at the microtubule plus ends is greatly impaired upon TACC depletion and depends on presence of the TACC domain. TACC-RNAi cells and TACC-RNAi cells expressing GFP-TACCdom were mixed, fixed with methanol and labeled with anti-CP224 (a, b). GFP-TACCdom expressing cells (arrowheads in a) can easily be identified due to their green fluorescent centrosomes. The typical staining pattern of CP224 at mictrotubule tips can only be observed in cells expressing GFP-TACCdom, while centrosomal CP224 can be seen in both cell types. c–e proves the microtubule tip localization of CP224 in wild-type cells stained with anti-CP224 (c, red in e) and anti-tubulin (d, green in e). In merged images DNA is shown in blue. Bar 2 μm