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Depletion of TACC does not impair mitotic CP224 localization. Wild-type cells (a, b, e, f, i, j) and TACC-RNAi cells (c, d, g, h, k, l) were fixed with methanol and labeled with anti-CP224 (grayscale images, red in merged images) and anti-tubulin (green in merged images). Mitotic stages are indicated on the left, cell types on top. In merged images DNA is shown in blue. Bar 2 μm
