Fig. 6.

RNAi depletion of TACC. Upon RNAi treatment TACC is no longer detectable in immunoblots (a). Depletion of TACC does not affect overall CP224 expression (b). After transfection of TACC-RNAi cells with GFP-TACCdom, the RNAi effect remained strong and no full-length TACC was detected (c). Western blots of whole cell lysates were stained with anti-TACC (c, upper panel in a), anti-CP224 (upper panel in b) and anti-actin (lower panel in a and b). Molecular weight is depicted on the left, strains used indicated on top of the respective lanes