Fig. 7.

Microtubule length is significantly reduced upon TACC RNAi and rescued by expression of the TACC domain as GFP fusion protein. Cell types indicated on top were fixed with glutaraldehyde and stained with anti-tubulin (e–h, green in a, b and red in c, d). Compared to wild-type cells, microtubule length is reduced in TACC-RNAi cells. Although GFP-TACCdom and GFP-CP224 expressed in TACC-RNAi cells both localize normally (green in c and d), only GFP-TACC-domain expression restores wild-type-like microtubule length in a large number of cells. The upper panel shows merged images of anti-tubulin, DNA (blue) and the respective GFP fusion proteins in c and d. Bar 2 μm