Fig. 9.

Astral microtubule formation is impaired upon TACC RNAi. Cell types indicated on top were fixed with glutaraldehyde and labeled with anti-tubulin (grayscale images and red in merged images) and anti-Cenp68 as a kinetochore marker (green in merged images a, c, e, g and j, l, n, p). Aster formation is heavily impaired upon TACC RNAi while spindle microtubules are unaffected. Expression of GFP-TACCdom in TACC-RNAi cells (green in merged images r, t, v, x) restores normal astral microtubule length. DNA is shown in blue, Bar 2 μm