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Microtubule length in wild-type and TACC mutant cells
| Cell line | Microtubule length μm (n) |
|---|---|
| Control cells (strain AX2) | 8.68 ± 2.59 (135) |
| TACC-RNAi | 6.17 ± 1.62 (132) |
| GFP-TACCdom in TACC-RNAi | 6.83 ± 2.38 (127) |
| GFP-CP224 in TACC-RNAi | 6.34 ± 1.89 (129) |
In order to assess microtubule length, the respective strains were fixed with glutaraldehyde and labeled with anti-tubulin. Evaluation of microtubule length was accomplished with ImageJ on maximum intensity projection images
