Fig. 4.

Experimental association of human candidate genes with cell division in MCF7 cell cultures. Genes were silenced in breast epithelial cancer cell cultures (MCF7 cells) using small interfering (si) RNA sequences. a Growth curves of the siRNA knocked-down MCF7 cultures, illustrating growth inhibition by knock-down of C14ORF21 and HEATR6. b Ploidy distributions of the STATIP1, C14ORF21, and HEATR6 knocked-down cultures in comparison with controls assessed by flow cytometry, illustrating a significant increased number of G2/M cells in C14ORF21 and HEATR6 knock-down cultures (P < 0.05 (n = 9) according to a t test). c Expression levels of cell cycle phase makers measured by Q-PCR, illustrating transcriptional upregulation of the G2/M marker genes CDK1, CyclinB1, and CyclinB2 in C14ORF21 and HEATR6 knock-down cell cultures, indicative for a transient G2 arrest