Skip to main content
. 2011 Jul 9;69(4):611–627. doi: 10.1007/s00018-011-0765-8

Fig. 2.

Fig. 2

Functional analysis of the Tlx3 promoter in ES-NPs and IMR32 cell lines: a Schematic of the Tlx3 promoter with different transcription factor-binding sites. b Tlx3-driven GFP expression cassette used to transfect ES-NPs. Since the level of expression of Tlx3 is low, we enhanced the visualization of Tlx3 expression using a Cre-lox construct, pTlx3 1310-CREM-CAG-EGFP. cd ES-NPs upon differentiation showed GFP expression indicating functional activity of the cloned Tlx3 promoter. e Luciferase activity in ES-NPs upon transient transfection of pTlx3-1310-Luc in differentiated ES-NPs showed a significant increase (p < 0.001) compared to control. f Schematic of Tlx3 promoter-GFP reporter construct used to study Tlx3 expression in the IMR32 cell line. gh Transient transfections with pTlx3-1310-EGFP construct showed GFP-positive cells, indicating Tlx3 expression in IMR32 cell line. i This was again confirmed by assaying Tlx3 promoter activity, which showed a significant increase (p < 0.001) in luciferase expression compared to the control. Significantly high promoter activation indicates a higher level of expression of Tlx3 in IMR32 cell line compared to ES-NPs. Data are expressed as mean ± SD of triplicates (n = 3) from three different experiments. Scale bar = 20 µM