Fig. 2a, b.

Phosphorylation status of mitochondrial Src in in vitro treatments. a Mitochondria, treated as described in the legend to Fig. 1, were Western blotted and immunodetected first with the anti-pY416-Src antibody, stripped and reprobed with the anti-pY527-Src antibody, stripped again and finally probed with an antibody to MnSOD as a loading control. b The intensity of pY416-Src and pY527-Src labeling was normalized over the MnSOD signal for each treatment. Data are representative of at least three experiments. Means that are not significantly different (P > 0.05) according to the Tukey test share the same letters