Fig. 4.

Effect of hsa-miR-128 on the apoptotic pathway proteins in HEK293T cells. Western blot analysis of Bax, p53, p-p53, Bcl-2, Bcl-xL, Bak and Bid performed on total cell extracts from untransfected HEK293T cells and HEK293T cells transfected with 2 or 4 μg p(128). β-actin was used a loading control. Data are representative of a typical experiment repeated three times with similar results. The protein band was quantified and normalized to β-actin. Bar graph represents the mean ± SEM, *p < 0.05 versus control. Normalized ID values represent normalized integrated densitometric values