Table 1.
A summary of the spectral peaks of absorbance and relative levels of opsin produced by wild-type (WT) and mutant mouse MWS photopigments, under chloride and chloride-free conditions, calculated by comparing the ratio of total protein absorbance (A280) to the normalised absorbance of opsin protein determined at the λmax (i.e. Aprotein/Aopsin)
| Opsin | +Cl− | −Cl− | ||||
|---|---|---|---|---|---|---|
| Peak (nm) | Ratio (Aprotein/Aopsin) | % of WT | Peak (nm) | Ratio (Aprotein/Aopsin) | % of WT | |
| WT (Y197/S308) | 511 | 7.2 | 100 | 514 | 14.2 | 50.7 |
| H197/S308 | 525 | 33.2 | 21.7 | No pigment | 86.2 | No pigment |
| Y197/A308 | 531 | 7.5 | 95.8 | 525 | 7.8 | 92.4 |
| H197/A308 | 551 | 14.9 | 48.1 | No pigment | 88.0 | No pigment |
Each resultant ratio was converted to a percentage of the wild-type ratio to produce a realtive measure of opsin-specific protein for comparison