Fig. 2.

Direct binding assays between Tetraodon CD25-like and IL-2/IL-15. a Purified fusion CD25-like, IL-2 and IL-15 were revealed by SDS-PAGE. M protein marker; lanes 1–2 GST-CD25-like and GST proteins, respectively; lanes 3–5 Trx-IL-2, Trx-IL-15 and Trx proteins, respectively. b Direct binding ELISAs were conducted for the detection of the interaction between solid-phase CD25-like with Trx fusion ligands. The fusion GST-CD25-like binding values minus the GST background values were exhibited here, and the background value of CD25-like and Trx tag is relatively low. The means of three experiments are presented for each set of experiments