Fig. 6.

Assessment of Tetraodon inflammatory bowel disease after depletion of CD25-like⁺ cells. a Flow cytometry analysis for CD4-2⁺CD25-like⁺ cells of a fish that was injected with 2 μg anti-CD25-like Ab for 6 weeks comparing with a control fish injected with 2 μg rabbit IgG. b The guts of these two fish were fixed in 10% formalin and processed for paraffin sectioning and, HE staining: 1 for control fish injected with rabbit IgG, 2 for fish injected with anti-CD25-like antibodies. c Detection of intestine IFN-γ and TNF-α 6 weeks after injection with anti-CD25-like antibodies by RT-PCR analyses: C for normal fish, 0 injection with 2 μg rabbit IgG, 1 injection with 0.5 μg anti-CD25-like antibodies, 2 injection with 2 μg anti-CD25-like antibodies, 3 injection with 5 μg anti-CD25-like antibodies, and the corresponding semi-quantitative analyses of these two genes relative to β-actin were shown in lower panel. d Weekly detection of intestine IFN-γ and TNF-α for injection with 2 μg anti-CD25-like antibodies by RT-PCR, and the corresponding semi-quantitative analyses are shown in the lower panel