Fig. 2.

Hands-off maintenance of an extrachromosomal C. elegans line on antibiotic-selective medium. An extrachromosomal C. elegans line carrying the NeoR nematode cassette and a _p myo-2::gfp reporter (worms carrying _p myo-2::gfp express GFP in the pharynx) amplified on a plate without G-418 (left) and on a plate supplemented with G-418 at the critical concentration (selective plate) (right). After several generations and in the absence of a selective agent, the extrachromosomal array is lost and transgenic animals are rare (left) while on selective plates wild-type worms are eliminated at the L1 stage and the transgenic population is enriched without manual maintenance